New Lyme Disease DNA Test For Detecting Early Lyme

There is now a more sensitive and specific test available for detecting EARLY Lyme disease at the Milford Hospital in Connecticut! This is a first of its kind DNA test developed by pathologist Sin Hang Lee, MD and his team. Detecting and treating Lyme early is of paramount importance. If you are treated in the early stages, you have a good chance of receiving treatment that will cure you, whereas if Lyme is not caught early, the infection has time to spread throughout the body, often resulting in chronic Lyme. (Note: most insurance providers cover this new DNA test, except for Aetna)

Patients and physicians interested in information on this DNA test may call George Poole, manager of Milford Medical Laboratory, at 203-876-4496.

Here is the abstract of the journal article covering the findings of this new DNA test for detecting EARLY Lyme disease.

Clin Pathol. 2010 Apr;133(4):569-76.

Increased sensitivity and specificity of Borrelia burgdorferi 16S ribosomal DNA detection.

Lee SH, Vigliotti VS, Vigliotti JS, Jones W, Pappu S.

Department of Pathology, Milford Hospital, CT, USA.

Abstract
The DNA of Borrelia burgdorferi spirochetes extracted by ammonium hydroxide was used as the template for nested polymerase chain reaction (PCR) amplification of the species-specific 16S ribosomal DNA (rDNA). The primers were those well known to be specific for signature sequence amplification of the B burgdorferi sensu lato 16S ribosomal RNA gene. The positive 293-base-pair nested PCR amplicon was subjected to routine direct automated Sanger sequencing. A 50-base sequence excised randomly from the sequencing electrophoretogram between the 2 nested PCR primer binding sites was sufficient for the Basic Local Alignment Search Tool (BLAST) analysis to validate the B burgdorferi sensu lato 16S rDNA without a reasonable doubt. Nested PCR increased the sensitivity of DNA detection by 100- to 1,000-fold. DNA sequence validation based on BLAST algorithms using the GenBank database practically eliminates any possibility of false-positive results due to molecular misidentification. This technology may be a valuable supplement to the current serologic tests for Lyme disease.

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2 comments for “New Lyme Disease DNA Test For Detecting Early Lyme

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