Dr. Eva Sapi’s Exciting New Lyme Cultures!

FROM: Lymediseaseresource.com

CALDA printed the following information in their blog recently. The article, HARDSCIENCEONLYME: Lyme Culture Made Easy, Antibiotic Effectiveness, and Mechanisms of Persistence written by CALDA’s President Lorraine Johnson can also be found on CALDA’s website http://www.lymedisease.org/:

Dr. Eva Sapi and colleagues have published an important study today, c E oeEvaluation of in-vitro antibiotic susceptibility of different morphological forms of Borrelia burgdorferi c E . I have included the link to the article, which is available free of charge at Dove Press, at the end of this post and encourage you to read it.

The study is broad ranging, but makes a significant contribution to Lyme research in a number of areas: culturing techniques, cyst and spirochete antibiotic susceptibility, and the role of biofilms in persistence.

The most important result of the research was the development of a new culture technique that dramatically increases the growth of Borrelia burgdorferi (Bb), the corkscrew-shaped bacteria (spirochete) that causes Lyme disease, making it easier to detect persistence of the bacteria. The new culture technique is also better able to grow the cyst form of the bacteria that is thought to be metabolically dormant.

One mechanism of persistence of Bb is believed to be its ability to convert from the spirochete form to the cyst form in vitro when presented with an unfavorable environment. This conversion would allow the bacteria to evade the immune system and resist destruction by antibiotics that rely on cell division for effectiveness. When the environmental conditions become more favorable, the organism can then revert back to the spirochete form.

The ability to easily culture the cyst form as well as the spirochete form of the bacteria permitted the researchers to determine the effect of various antibiotics on each form of the bacteria. Some antibiotics decreased the number of spirochetes but increased the number of cysts, while other antibiotics reduced both spirochetes and cysts, but at different rates.

Finally, the researchers were able to develop biofilm colonies of the Lyme bacteria. These colonies are made up of bacterial clusters coated in slime, and they have been shown to promote persistent infection in other diseases. The effectiveness of antibiotics for Bb in biofilm colonies was greatly reduced, with viable organisms detected in 70-85% of the biofilm colonies.

In summary, the study achieved an important new advance in culture techniques for Bb, demonstrated varying sensitivity of the cyst and spirochete form of Bb to different antibiotics, and confirmed mechanisms of persistence of Bb by converting from spirochete to cyst form and by forming biofilm colonies that protect the organism from antibiotics.

The research was supported by grants from the California Lyme Disease Association, Turn the Corner Foundation and the University of New Haven. The full text of the article is available without charge at Infections and Drug Resistance.


3 comments for “Dr. Eva Sapi’s Exciting New Lyme Cultures!

  1. Beverly DeMartini
    May 28, 2013 at 1:00 pm

    My son (40 yrs) was tested for Lyme disease and when negative moved on to other causes for his symptoms. Spent a week at the Mayo Clinic in Jacksonville Fl and they found nothing wrong with him. Finally his neurologist diagnosed him with myasthenia gravis.

    He spent a couple years trying to get an answer. From what I read the tests done for lyme disease are not
    reliable and I’m not sure any test other than a standard one was given.

    He’s been on medication for about a year. He can’t tolerate predozone. I think he is getting worse.

    Is there anywhere in the Chicago area where he can get tested in depth for Lyme disease? And at what cost? Currently he has insurance, but if it doesn’t cover the test I’d like to have a idea of out of pocket.

    Would really appreciate getting some information
    Beverly DeMartini

    • August 3, 2014 at 10:58 am

      Thank-you, what a wonderful altrcie, I have had complete right hip replacement and am facing a possible left hip surgery. I am working with a group doing tapping or EFT and learning to remove the doubts and refraze our thinking. Since my doctor let me get back on my bicycle I have been working on my diet and exercising with working myself up to riding approximately 20 miles a day for 5 days.. I have lost 8 inches and approx. 30#+. Lots of tapping for emotional situations that have come up and a knowing that I know I can reach my goal of 14 inches and 90#s.. Thanks again Karen

    • August 4, 2014 at 7:27 pm

      Hi Everyone, You are welcome! I’m glad the vodeis have been of benefit. Sometimes I wonder how much people are really getting out of them so it helps to get positive feedback. Also, if there is a particular subject anyone would like to hear about please feel free to shoot me suggestions. Sending healing prayers your way, Connie

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